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https://hdl.handle.net/20.500.11851/6861
Title: | Impact of Preterm Birth on the Cellular Characteristics of Neonatal Buccal Cells | Authors: | Dönmez, Hanife Güler Çelik, Hasan Tolga Kayki, Gözdem Yiğit, Şule Yurdakök, Murat Çakar, Ayşe Nur Beksaç, Mehmet Sinan |
Keywords: | buccal smear cytomorphometry Feulgen micronucleus Papanicolaou preterm birth |
Publisher: | Wiley | Abstract: | Objective To demonstrate the impact of preterm birth on the cytological, cytomorphometrical, and nuclear parameters of neonatal buccal smears. Methods This study consisted of Early Preterm Neonates (EPN; <= 34th gestational week [gw]; n = 36), Late Preterm Neonates (LPN; 34th to <37th gw; n = 46), and Term Neonates (control; >= 37th gw; n = 56). Cytological evaluation and buccal cytome assay were performed using Papanicolaou and Feulgen methods, respectively. Results Cytological evaluation demonstrated that smear background was cleaner (P < .05) and there were less macrophages in the control group (P < .001). Cyto-morphometric analysis showed that the measurements of nuclear diameter, nuclear area, and nucleus-to-cytoplasm ratio were higher in the preterm (EPN and LPN) versus the control groups (P = .016, P < .001, and P < .001, respectively). We also demonstrated that staining intensity of the nucleus and cytoplasm were less intense in the EPN and LPN groups (P < .001). There was no statistically significant difference between the EPN and LPN groups for any parameters (P > .05). Buccal cytome assay showed that nuclear buds were more prevalent in term newborns compared to preterm neonates (P < .001). Conclusions Morphological and cytological properties of neonatal buccal cells are influenced by preterm birth status, and buccal smears may be used as a tool to detect biological markers of neonatal health problems. | URI: | https://doi.org/10.1111/cyt.12992 https://hdl.handle.net/20.500.11851/6861 |
ISSN: | 0956-5507 1365-2303 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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