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https://hdl.handle.net/20.500.11851/6420
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Demiralp, Duygu Özel | - |
dc.contributor.author | Peker, Selen | - |
dc.contributor.author | Turgut, Burhan | - |
dc.contributor.author | Akar, Nejat | - |
dc.date.accessioned | 2021-09-11T15:36:24Z | - |
dc.date.available | 2021-09-11T15:36:24Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.issn | 1862-8346 | - |
dc.identifier.issn | 1862-8354 | - |
dc.identifier.uri | https://doi.org/10.1002/prca.201200010 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.11851/6420 | - |
dc.description.abstract | Purpose Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1DE) may reveal qualitative or quantitative defects in red blood cell (RBC) membrane proteins, two-dimensional gel electrophoresis (2DE) can be used for determination of the protein changes caused by the disease process in a relatively high-throughput manner, because it permits an analysis of thousands of modified or unmodified proteins simultaneously. The principal aim of this study was to compare hereditary elliptocytosis (HE), hereditary spherocytosis (HS), and control RBC membrane protein profiles and identify proteins as a clinical marker by the sensitive methods. Experimental design RBC membrane proteins of HE, HS, and control groups were compared by 2DE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS analysis was obtained using peptide mass fingerprint for protein identification. Results Twenty proteins were identified with peptide mass fingerprint analysis and different expression levels were found in HE, HS, and controls for some proteins that includes three biomarker proteins (ankyrin, spectrin, band 3) that may have prognostic or predictive importance. Conclusions and clinical relevance 2DE of RBC proteins is a potentially valuable method for studying heritable disorders such as HE and HS. By identifying a deficiency in membrane proteins associated with the RBC cytoskeleton, the diagnosis of HE and HS can be confirmed. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) | en_US |
dc.description.sponsorship | This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley-V C H Verlag Gmbh | en_US |
dc.relation.ispartof | Proteomics Clinical Applications | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Hereditary elliptocytosis | en_US |
dc.subject | Hereditary spherocytosis | en_US |
dc.subject | MALDI-TOF MS | en_US |
dc.subject | Two-dimensional gel electrophoresis | en_US |
dc.title | Comprehensive Identification of Erythrocyte Membrane Protein Deficiency by 2d Gel Electrophoresis Based Proteomic Analysis in Hereditary Elliptocytosis and Spherocytosis | en_US |
dc.type | Article | en_US |
dc.department | Faculties, School of Medicine, Department of Internal Medical Sciences | en_US |
dc.department | Fakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü | tr_TR |
dc.identifier.volume | 6 | en_US |
dc.identifier.issue | 7-8 | en_US |
dc.identifier.startpage | 403 | en_US |
dc.identifier.endpage | 411 | en_US |
dc.authorid | 0000-0002-1798-7951 | - |
dc.identifier.wos | WOS:000308093000010 | en_US |
dc.identifier.scopus | 2-s2.0-84865609351 | en_US |
dc.institutionauthor | Turgut, Burhan | - |
dc.identifier.pmid | 22807418 | en_US |
dc.identifier.doi | 10.1002/prca.201200010 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.scopusquality | Q3 | - |
item.openairetype | Article | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | Dahili Tıp Bilimleri Bölümü / Department of Internal Medical Sciences PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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