Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.11851/2884
Title: | Nanopillared Chitosan/Gelatin Films: a Biomimetic Approach for Improved Osteogenesis | Authors: | Altuntaş, Sevde Dhaliwal, Harkiranpreet K. Bassous, Nicole J. Radwan, Ahmed E. Alpaslan, Pınar Webster, Thomas Büyükserin, Fatih Mansoor, Amiji |
Keywords: | nanopillared chitosan/gelatin film osteogenic differentiation mineralization |
Publisher: | American Chemical Society | Source: | Altuntas, S., Dhaliwal, H. K., Bassous, N. J., Radwan, A. E., Alpaslan, P., Webster, T., ... & Amiji, M. (2019). Nanopillared Chitosan/Gelatin Films: A Biomimetic Approach for Improved Osteogenesis. ACS Biomaterials Science & Engineering, 5(9), 4311-4322. | Abstract: | Biomimicry strategies, inspired from natural organization of living organisms, are being widely used in the design of nanobiomaterials. Particularly, nonlithographic techniques have shown immense potential in the facile fabrication of nanostructured surfaces at large-scale production. Orthopedic biomaterials or coatings possessing extracellular matrix-like nanoscale features induce desirable interactions between the bone tissue and implant surface, also known as osseointegration. In this study, nanopillared chitosan/gelatin (C/G) films were fabricated using nanoporous anodic alumina molds, and their antibacterial properties as well as osteogenesis potential were analyzed by comparing to the flat C/G films and tissue culture polystyrene as controls. In vitro analysis of the expression of RUNX2, osteopontion, and osteocalcin genes for mesenchymal stem cells as well as osteoblast-like Saos-2 cells was found to be increased for the cells grown on nano C/G films, indicating early-stage osteogenic differentiation. Moreover, the mineralization tests (quantitative calcium analysis and alizarin red staining) showed that nanotopography significantly enhanced the mineralization capacity of both cell lines. This work may provide a new perspective of biomimetic surface topography fabrication for orthopedic implant coatings with superior osteogenic differentiation capacity and fast bone regeneration potential. | URI: | https://hdl.handle.net/20.500.11851/2884 https://pubs.acs.org/doi/10.1021/acsbiomaterials.9b00426 |
ISSN: | 2373-9878 |
Appears in Collections: | Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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